Rat renal cortical OAT1 and OAT3 exhibit gender differences determined by both androgen stimulation and estrogen inhibition
- PMID: 15010355
- DOI: 10.1152/ajprenal.00029.2004
Rat renal cortical OAT1 and OAT3 exhibit gender differences determined by both androgen stimulation and estrogen inhibition
Abstract
In rats, the secretion of p-aminohippurate (PAH) by the kidney is higher in males (M) than in females (F). The role of the major renal PAH transporters, OAT1 and OAT3, in the generation of these gender differences, as well as the responsible hormones and mechanisms, has not been clarified. Here we used various immunocytochemical methods to study effects of gender, gonadectomy, and treatment with sex hormones on localization and abundance of OAT1 and OAT3 along the rat nephron. Both transporters were localized to the basolateral membrane: OAT1 was strong in proximal tubule S2 and weak in the S3 segments, whereas OAT3 was stained in proximal tubule S1 and S2 segments, thick ascending limb, distal tubule, and in principal cells along the collecting duct. Gender differences in the expression of both transporters in adult rats (M > F) were observed only in the cortical tubules. OAT1 in the cortex was strongly reduced by castration in adult M, whereas the treatment of castrated M with testosterone, estradiol, or progesterone resulted in its complete restitution, further depression, or partial restitution, respectively. In adult F, ovariectomy weakly increased, whereas estradiol treatment of ovariectomized F strongly decreased, the expression of OAT1. The expression of OAT3 in the M and F cortex largely followed a similar pattern, except that ovariectomy and progesterone treatment showed no effect, whereas in other tissue zones gender differences were not observed. In prepubertal rats, the expression of OAT1 and OAT3 in the kidney cortex was low and showed no gender differences. Our data indicate that gender differences in the rat renal cortical OAT1 and OAT3 (M > F) appear after puberty and are determined by both a stimulatory effect of androgens (and progesterone in the case of OAT1) and an inhibitory effect of estrogens.
Similar articles
-
Renal expression of organic anion transporter OAT2 in rats and mice is regulated by sex hormones.Am J Physiol Renal Physiol. 2007 Jan;292(1):F361-72. doi: 10.1152/ajprenal.00207.2006. Epub 2006 Aug 1. Am J Physiol Renal Physiol. 2007. PMID: 16885152
-
Upregulation of rat renal cortical organic anion transporter (OAT1 and OAT3) expression in response to ischemia/reperfusion injury.Am J Nephrol. 2008;28(5):772-83. doi: 10.1159/000129073. Epub 2008 Apr 28. Am J Nephrol. 2008. PMID: 18441523
-
Downregulation of organic anion transporters OAT1 and OAT3 correlates with impaired secretion of para-aminohippurate after ischemic acute renal failure in rats.Am J Physiol Renal Physiol. 2007 May;292(5):F1599-605. doi: 10.1152/ajprenal.00473.2006. Epub 2007 Jan 23. Am J Physiol Renal Physiol. 2007. PMID: 17244891
-
Transport of organic anions across the basolateral membrane of proximal tubule cells.Rev Physiol Biochem Pharmacol. 2003;146:95-158. doi: 10.1007/s10254-002-0003-8. Epub 2003 Jan 30. Rev Physiol Biochem Pharmacol. 2003. PMID: 12605306 Review.
-
The molecular and cellular physiology of basolateral organic anion transport in mammalian renal tubules.Biochim Biophys Acta. 2003 Dec 30;1618(2):185-93. doi: 10.1016/j.bbamem.2003.08.015. Biochim Biophys Acta. 2003. PMID: 14729155 Review.
Cited by
-
Unexpected Nephrotoxicity in Male Ablactated Rats Induced by Cordyceps militaris: The Involvement of Oxidative Changes.Evid Based Complement Alternat Med. 2013;2013:786528. doi: 10.1155/2013/786528. Epub 2013 Feb 24. Evid Based Complement Alternat Med. 2013. PMID: 23533520 Free PMC article.
-
Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic.Front Pharmacol. 2021 Apr 13;12:643972. doi: 10.3389/fphar.2021.643972. eCollection 2021. Front Pharmacol. 2021. PMID: 33927623 Free PMC article. Review.
-
Multi-level analysis of organic anion transporters 1, 3, and 6 reveals major differences in structural determinants of antiviral discrimination.J Biol Chem. 2008 Mar 28;283(13):8654-63. doi: 10.1074/jbc.M708615200. Epub 2008 Jan 3. J Biol Chem. 2008. PMID: 18174163 Free PMC article.
-
The liver and kidney expression of sulfate anion transporter sat-1 in rats exhibits male-dominant gender differences.Pflugers Arch. 2009 Apr;457(6):1381-92. doi: 10.1007/s00424-008-0611-5. Epub 2008 Nov 11. Pflugers Arch. 2009. PMID: 19002488
-
Hyperuricemia and risk of increased arterial stiffness in healthy women based on health screening in Korean population.PLoS One. 2017 Jun 30;12(6):e0180406. doi: 10.1371/journal.pone.0180406. eCollection 2017. PLoS One. 2017. PMID: 28666027 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources