ELABORATION OF BODY PLAN AND PHASE CHANGE DURING DEVELOPMENT OF ACETABULARIA: How Is the Complex Architecture of a Giant Unicell Built?

Abstract

While uninucleate and unicellular, Acetabularia acetabulum establishes and maintains functionally and morphologically distinct body regions and executes phase changes like those in vascular plants. Centimeters tall at maturity, this species has allowed unusual experimental approaches. Amputations revealed fates of nucleate and enucleate portions from both wild type and mutants. Historically, graft chimeras between nucleate and enucleate portions suggested that morphological instructions were supplied by the nucleus but resided in the cytoplasm and could be expressed interspecifically. Recently, graft chimeras enabled rescue of mutants arrested in vegetative phase. Since the 1930s, when Acetabularia provided the first evidence for the existence of mRNAs, a dogma has arisen that it uses long-lived mRNAs to effect morphogenesis. While the evidence favors translational control, the postulated mRNAs have not been identified, and the mechanism of morphogenesis remains unknown. Amenable to biochemistry, physiology, and both classical and molecular genetics, Acetabularia may contribute yet new insights into plant development and morphogenesis.