doi: 10.1038/358560a0.
A small metalloribozyme with a two-step mechanism
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- PMID: 1501711
- DOI: 10.1038/358560a0
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A small metalloribozyme with a two-step mechanism
Nature.
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Abstract
An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.
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