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. 1992 Aug 13;358(6387):560-3.
doi: 10.1038/358560a0.

A small metalloribozyme with a two-step mechanism

T Pan  1 , O C Uhlenbeck
Affiliations

A small metalloribozyme with a two-step mechanism

T Pan et al. Nature. .

Abstract

An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.

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