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. 2003 Nov;24(11):1723-31.
doi: 10.1016/j.peptides.2003.07.025.

In vitro and in vivo antimicrobial activity of two alpha-helical cathelicidin peptides and of their synthetic analogs

Monica Benincasa  1 , Barbara Skerlavaj, Renato Gennaro, Antonio Pellegrini, Margherita Zanetti
Affiliations

In vitro and in vivo antimicrobial activity of two alpha-helical cathelicidin peptides and of their synthetic analogs

Monica Benincasa et al. Peptides. 2003 Nov.

Abstract

Two alpha-helical antimicrobial peptides (BMAP-27 and -28) and four synthetic analogs were compared for in vitro and in vivo antimicrobial efficacy. All peptides proved active in vitro at micromolar concentrations against a range of clinical isolates, including antibiotic-resistant strains. BMAP-27 and two analogs were more effective towards Gram-negative, and BMAP-28 towards Gram-positive organisms. In addition, BMAP-28 provided some protection in vitro against human herpes simplex virus type 1 (HSV-1). The parent peptides and mBMAP-28 analog protected mice from lethal i.p. infections in an acute peritonitis model at peptide doses significantly lower than those toxic to the animals, suggesting a satisfactory therapeutic index.

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Figures

Fig. 1
Fig. 1
Distribution of MIC values of BMAP peptides for Gram-positive clinical isolates. (A) Enterococcus faecalis (10 strains) and (B) Staphylococcus aureus (10 strains). The hatched part of bars refers to the vancomycin-resistant E. faecalis strains (A, four in total) or methicillin-resistant S. aureus strains (B, five in total).
Fig. 2
Fig. 2
Distribution of MIC values of BMAP peptides for Gram-negative clinical isolates. (A) Pseudomonas aeruginosa (10 strains) and (B) Acinetobacter baumanni.
See this image and copyright information in PMC

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