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Comment

. 2004 Mar 23;101(12):3995-6.

doi: 10.1073/pnas.0400671101. Epub 2004 Mar 15.

Membrane proteins: a new method enters the fold

James U Bowie¹

Affiliations

Affiliation

¹ Dept. of Chemistry and Biochemistry, UCLA-Dept. of Energy Center for Genomics and Proteomics, Molecular Biology Institute, University of California-Los Angeles, Boyer Hall, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA. bowie@mbi.ucla.edu

PMID: 15024105
PMCID: PMC384682
DOI: 10.1073/pnas.0400671101

Comment

Membrane proteins: a new method enters the fold

James U Bowie. Proc Natl Acad Sci U S A. 2004.

. 2004 Mar 23;101(12):3995-6.

doi: 10.1073/pnas.0400671101. Epub 2004 Mar 15.

Author

James U Bowie¹

Affiliation

¹ Dept. of Chemistry and Biochemistry, UCLA-Dept. of Energy Center for Genomics and Proteomics, Molecular Biology Institute, University of California-Los Angeles, Boyer Hall, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA. bowie@mbi.ucla.edu

PMID: 15024105
PMCID: PMC384682
DOI: 10.1073/pnas.0400671101

No abstract available

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Figures

Fig. 1. — Fig. 1.
Membrane protein unfolding. For helical proteins, shown on the left, tertiary structure can be lost within the membrane environment, leaving stable transmembrane helices. Thus, the insertion of helices and folding (helix packing) can be separated. For β-barrel proteins, secondary structure should not be independently stable in the membrane. Thus, folding and insertion are likely to be coupled. The free energy of this folding and insertion equilibrium has now been measured by Hong and Tamm (2). They were able to study the effects of bilayer properties on this equilibrium. A mismatch between the hydrophobic width of the protein (green region) and the bilayer can induce distortions in either the protein or the bilayer. Cone-shaped lipids (yellow head group) lead to increased packing pressure in the center of the bilayer and decreased packing pressure at the head groups, which can be alleviated by the insertion of an hourglass-shaped protein.

See this image and copyright information in PMC

Comment on

Elastic coupling of integral membrane protein stability to lipid bilayer forces.
Hong H, Tamm LK. Hong H, et al. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4065-70. doi: 10.1073/pnas.0400358101. Epub 2004 Feb 27. Proc Natl Acad Sci U S A. 2004. PMID: 14990786 Free PMC article.

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