Review
[Strategy of production and utilization of immortal cells]
[Article in
Russian]
Affiliations
- PMID: 15027207
Item in Clipboard
Review
[Strategy of production and utilization of immortal cells]
[Article in
Russian]
Ontogenez.
2004 Jan-Feb.
Abstract
The current methods of production of conditionally immortal cells in vivo and in vitro have been considered, including the method based on transgenesis of animals. Examples are given for utilization of conditionally immortal cells obtained in vivo from tissues of transgenic mice and rats carrying the gene of mutant T-antigen tsA58 SV40. The recent studies were analyzed, which concern the investigation and utilization of embryonic and regional stem cells, as well as immortal cells obtained through transfection of the recombinant construct of telomerase gene into human cells. The main problems of cell biotechnology are discussed.
Similar articles
-
Establishment and characterization of conditionally immortalized endothelial cell lines from the thoracic duct and inferior vena cava of tsA58/EGFP double-transgenic rats.Cell Tissue Res. 2006 Dec;326(3):749-58. doi: 10.1007/s00441-006-0229-x. Epub 2006 Jun 14. Cell Tissue Res. 2006. PMID: 16773315
-
A new in vitro model for blood-cerebrospinal fluid barrier transport studies: an immortalized choroid plexus epithelial cell line derived from the tsA58 SV40 large T-antigen gene transgenic rat.Adv Drug Deliv Rev. 2004 Oct 14;56(12):1875-85. doi: 10.1016/j.addr.2004.07.013. Adv Drug Deliv Rev. 2004. PMID: 15381338 Review.
-
Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse.FEBS J. 2008 May;275(9):1988-98. doi: 10.1111/j.1742-4658.2008.06353.x. Epub 2008 Mar 17. FEBS J. 2008. PMID: 18355322
-
Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein.BMC Biotechnol. 2005 Jul 4;5:20. doi: 10.1186/1472-6750-5-20. BMC Biotechnol. 2005. PMID: 15996270 Free PMC article.
-
Making stem cell lines suitable for transplantation.Cell Transplant. 2007;16(2):101-15. Cell Transplant. 2007. PMID: 17474292 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical