Review
doi: 10.1016/0968-0004(92)90407-z.
Ribotoxin recognition of ribosomal RNA and a proposal for the mechanism of translocation
Affiliations
- PMID: 1502728
- DOI: 10.1016/0968-0004(92)90407-z
Item in Clipboard
Review
Ribotoxin recognition of ribosomal RNA and a proposal for the mechanism of translocation
Trends Biochem Sci.
1992 Jul.
Abstract
The ribotoxins alpha-sarcin and ricin catalyse covalent modifications in adjacent nucleotides in 28S rRNA, yet the elements of nucleic acid structure that they recognize are not only different but incompatible. This suggests that this ribosomal domain (which in two dimensions is a seven-base-pair helical stem and a 17-member single-stranded loop) has alternate conformers. Since the domain is involved in binding of aminoacyl-tRNA and GTP hydrolysis, we propose that the switch between the two configurations, perhaps initiated by the binding of elongation factors, plays a role in translocation.
Similar articles
-
The ribosomal RNA identity elements for ricin and for alpha-sarcin: mutations in the putative CG pair that closes a GAGA tetraloop.Nucleic Acids Res. 1994 Feb 11;22(3):321-4. doi: 10.1093/nar/22.3.321. Nucleic Acids Res. 1994. PMID: 8127668 Free PMC article.
-
Ribosomal RNA identity elements for recognition by ricin and by alpha-sarcin: mutation in the putative CG pair that closes a GAGA tetraloop.Nucleic Acids Symp Ser. 1993;(29):165-6. Nucleic Acids Symp Ser. 1993. PMID: 8247752
-
Characterization of in vitro and in vivo mutations in non-conserved nucleotides in the ribosomal RNA recognition domain for the ribotoxins ricin and sarcin and the translation elongation factors.J Mol Biol. 1999 Jan 15;285(2):567-80. doi: 10.1006/jmbi.1998.2337. J Mol Biol. 1999. PMID: 9878430
-
RNA recognition and base flipping by the toxin sarcin.J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):93-6. doi: 10.1107/s0909049503023896. Epub 2003 Nov 28. J Synchrotron Radiat. 2004. PMID: 14646144 Review.
-
[The mechanisms of action of the cytotoxic proteins, alpha-sarcin and ricin A-chain on eukaryotic ribosomes].Seikagaku. 1989 Oct;61(10):1209-23. Seikagaku. 1989. PMID: 2693549 Review. Japanese. No abstract available.
Cited by
-
Similarities between Argonautes and the alpha-sarcin-like ribotoxins: Implications for microRNA action.Protein Sci. 2010 Jun;19(6):1272-8. doi: 10.1002/pro.391. Protein Sci. 2010. PMID: 20512980 Free PMC article.
-
The ribosomal RNA identity elements for ricin and for alpha-sarcin: mutations in the putative CG pair that closes a GAGA tetraloop.Nucleic Acids Res. 1994 Feb 11;22(3):321-4. doi: 10.1093/nar/22.3.321. Nucleic Acids Res. 1994. PMID: 8127668 Free PMC article.
-
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process.EMBO J. 2002 Jul 1;21(13):3557-67. doi: 10.1093/emboj/cdf326. EMBO J. 2002. PMID: 12093756 Free PMC article.
-
Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA.Mol Cell Biochem. 2001 Jul;223(1-2):117-21. doi: 10.1023/a:1017914413081. Mol Cell Biochem. 2001. PMID: 11681712
-
Visualization of elongation factor G on the Escherichia coli 70S ribosome: the mechanism of translocation.Proc Natl Acad Sci U S A. 1998 May 26;95(11):6134-8. doi: 10.1073/pnas.95.11.6134. Proc Natl Acad Sci U S A. 1998. PMID: 9600930 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
