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Comparative Study
. 2004 Aug;31(8):1097-104.
doi: 10.1007/s00259-004-1486-0. Epub 2004 Mar 17.

68Ga-labelled DOTA-derivatised peptide ligands

G-J Meyer  1 , H Mäcke, J Schuhmacher, W H Knapp, M Hofmann
Affiliations
Comparative Study

68Ga-labelled DOTA-derivatised peptide ligands

G-J Meyer et al. Eur J Nucl Med Mol Imaging. 2004 Aug.

Abstract

68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of 68Ge/68Ga generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the 68Ga eluate as well as labelling and purification steps for peptides, and can be used with different 68Ge/68Ga generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.

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