Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Mar 25;428(6981):427-31.
doi: 10.1038/nature02370.

A resource for large-scale RNA-interference-based screens in mammals

Patrick J Paddison  1 , Jose M Silva, Douglas S Conklin, Mike Schlabach, Mamie Li, Shola Aruleba, Vivekanand Balija, Andy O'Shaughnessy, Lidia Gnoj, Kim Scobie, Kenneth Chang, Thomas Westbrook, Michele Cleary, Ravi Sachidanandam, W Richard McCombie, Stephen J Elledge, Gregory J Hannon
Affiliations

A resource for large-scale RNA-interference-based screens in mammals

Patrick J Paddison et al. Nature. .

Abstract

Gene silencing by RNA interference (RNAi) in mammalian cells using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) has become a valuable genetic tool. Here, we report the construction and application of a shRNA expression library targeting 9,610 human and 5,563 mouse genes. This library is presently composed of about 28,000 sequence-verified shRNA expression cassettes contained within multi-functional vectors, which permit shRNA cassettes to be packaged in retroviruses, tracked in mixed cell populations by means of DNA 'bar codes', and shuttled to customized vectors by bacterial mating. In order to validate the library, we used a genetic screen designed to report defects in human proteasome function. Our results suggest that our large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery.

PubMed Disclaimer

Comment in

Publication types

MeSH terms

Substances