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. 2004 Apr;48(4):1365-8.
doi: 10.1128/AAC.48.4.1365-1368.2004.

Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry

Marie Claude Barc  1 , François Bourlioux, Lionel Rigottier-Gois, Céline Charrin-Sarnel, Claire Janoir, Hélène Boureau, Joël Doré, Anne Collignon
Affiliations

Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry

Marie Claude Barc et al. Antimicrob Agents Chemother. 2004 Apr.

Abstract

Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.

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Figures

FIG. 1.
FIG. 1.
Quantification of predominant groups of bacteria with FISH and flow cytometry detection using group-specific 16S rRNA oligonucleotide probes in two groups of human fecal flora-associated mice challenged with amoxicillin-clavulanic acid from day 1 to day 7 for the assay group (▴) and with saline from day 1 to day 7 for the control group (▪). (A) Total anaerobic flora with the EUB 338 FITC probe. (B) C. coccoides-E. rectale group with the Erec 482 probe. (C) Bacteroides-Porphyromonas-Prevotella group with the Bacto 1080 probe. (D) Enterobacteriaceae group with the Enter 1432 probe. The data are means ± standard deviations of the means. An asterisk indicates that the P value is <0.05 between the two groups.
See this image and copyright information in PMC

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