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. 2004 Apr;48(4):1426-9.
doi: 10.1128/AAC.48.4.1426-1429.2004.

Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand

Alan Brockman  1 , Sittaporn Singlam, Lucy Phiaphun, Sornchai Looareesuwan, Nicholas J White, François Nosten
Affiliations

Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand

Alan Brockman et al. Antimicrob Agents Chemother. 2004 Apr.

Erratum in

  • Antimicrob Agents Chemother. 2004 Aug;48(8):3214

Abstract

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

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Figures

FIG. 1.
FIG. 1.
Differences between responses to artesunate in vitro plotted against their means with 95% limits of agreement.
FIG. 2.
FIG. 2.
Differences between responses to atovaquone in vitro plotted against their means with 95% limits of agreement.
See this image and copyright information in PMC

References

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