Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Jul 25;163(3):335-8.
doi: 10.1007/BF00271963.

In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli

M Holsters, B Silva, F Van Vliet, J P Hernalsteens, C Genetello, M Van Montagu, J Schell

In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli

M Holsters et al. Mol Gen Genet. .

Abstract

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Bacteriol. 1971 Dec;108(3):1244-9 - PubMed
    1. J Mol Biol. 1972 Mar 28;65(2):259-72 - PubMed
    1. Nature. 1977 Feb 10;265(5594):560-1 - PubMed
    1. J Bacteriol. 1975 Jul;123(1):255-64 - PubMed
    1. Nature. 1977 Feb 10;265(5594):561-3 - PubMed

LinkOut - more resources