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. 2004 Spring:113-116:433-45.
doi: 10.1385/abab:114:1-3:433.

Enzymatic synthesis of monolaurin

Carla C B Pereira  1 , Mônica A P da Silva, Marta A P Langone
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Enzymatic synthesis of monolaurin

Carla C B Pereira et al. Appl Biochem Biotechnol. 2004 Spring.

Abstract

The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55 degrees C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.

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