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. 2004 May;48(5):360-3.
doi: 10.1007/s00284-003-4211-5.

Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga

Antonio Ortega-Rivas  1 , Jacob Lorenzo-Morales, Enrique Martinez-Carretero, Mercedes Villa Lloberas, Basilio Valladares Hernández, Antonio del Castillo-Remiro
Affiliations

Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga

Antonio Ortega-Rivas et al. Curr Microbiol. 2004 May.

Abstract

Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.

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