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. 2004 Apr;42(4):1542-6.
doi: 10.1128/JCM.42.4.1542-1546.2004.

Evaluation of the automated phoenix system for potential routine use in the clinical microbiology laboratory

J-L Donay  1 , D Mathieu, P Fernandes, C Prégermain, P Bruel, A Wargnier, I Casin, F X Weill, P H Lagrange, J L Herrmann
Affiliations

Evaluation of the automated phoenix system for potential routine use in the clinical microbiology laboratory

J-L Donay et al. J Clin Microbiol. 2004 Apr.

Abstract

A comparative study was designed to evaluate the identification (ID) and antimicrobial susceptibility testing (AST) performances of the BD Phoenix Automated Microbiology System (Becton Dickinson Diagnostic Systems [BD], Pont de Claix, France). A total of 305 single clinical isolates were collected, and comparisons were made with routine manual methods in use in our microbiology laboratories. The percentages of correct IDs were 93.3, 89.4, 91.8, and 85.7% for enterobacteria, nonfermenting gram-negative bacilli, staphylococci, and streptococci-enterococci, respectively. The median ID time was 3 h, and the median time for AST was 10 h 30 min. AST results showed variable percentages of errors for the different antibiotics. None of the enterobacteria and 0.3% of Pseudomonas aeruginosa isolates showed a very major error (VME). Only one strain of Staphylococcus aureus showed a VME with oxacillin. We demonstrate here the efficiency of the Phoenix system, which can be used for the majority of strains encountered in a university-based laboratory, for ID and AST.

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References

    1. Barry, J., A. Brown, V. Ensor, U. Lakhani, D. Petts, C. Warren, and T. Winstanley. 2003. Comparative evaluation of the VITEK 2 Advanced expert system (AES) in five UK hospitals. J. Antimicrob. Chemother. 51:1191-1202. - PubMed
    1. Bouvet, P. J. M., and P. A. D. Grimont. 1986. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffi. Int. J. Syst. Bacteriol. 36:228-240.
    1. Brisse, S., S. Stefani, J. Verhoef, A. Van Belkum, P. Vandamme, and W. Goessens. 2002. Comparative evaluation of the BD Phoenix and VITEK 2 automated instruments for identification of isolates of the Burkholderia cepacia complex. J. Clin. Microbiol. 40:1743-1748. - PMC - PubMed
    1. Carret, C., J. D. Cavallo, H. Chardon, C. Chidiac, P. Choutet, P. Courvalin, H. Dabernat, H. Drugeon, L. Dubreuil, F. Goldstein, V. Jarlier, R. Leclercq, M. H. Nicolas-Chanoine, A. Philippon, C. Quentin, B. Rouveix, J. Sirot, and J. C. Soussy. 2003. Comite de l'Antibiogramme de la Societe Francaise de Microbiologie report 2003. Int. J. Antimicrob. Agents 21:364-391. - PubMed
    1. Cavallo, J. D., F. Leblanc, R. Fabre, A. Fourticq-Esqueöute, and the GERPB. 2001. Survey of the antibiotic sensitivity of Pseudomonas aeruginosa in France and the distribution of beta-lactam resistance mechanisms: the GERPB 1999 study. Pathol. Biol. 49:534-539. - PubMed

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