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. 2004 Apr;42(4):1739-41.
doi: 10.1128/JCM.42.4.1739-1741.2004.

DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans

Gannavaram Sreenivas  1 , B V Subba Raju, Ruchi Singh, Angamuthu Selvapandiyan, Robert Duncan, Dwijen Sarkar, Hira L Nakhasi, Poonam Salotra
Affiliations

DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans

Gannavaram Sreenivas et al. J Clin Microbiol. 2004 Apr.

Abstract

Leishmania donovani in India causes visceral infection (kala-azar) and dermal infection (post-kala-azar dermal leishmaniasis). We report here the identification of polymorphism in a well-defined genetic locus among the Leishmania parasites causing the visceral and dermal manifestations, in a comparison of 15 post-kala-azar dermal leishmaniasis and 12 kala-azar patient isolates.

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Figures

FIG. 1.
FIG. 1.
Southern blot analysis of representative isolates from the three groups. Genomic DNA (5 μg) of L. donovani from groups K, P, and M was digested for 16 h at 37°C with MvaI (4 U/μg of DNA; MBI Fermentas) and resolved on a 1% agarose gel, and blots were probed with 32P-labeled LdP13 and visualized by autoradiography. The numbers below the lanes indicate the numbers of isolates falling in the respective categories.
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