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. 2004 Apr;42(4):1797-9.
doi: 10.1128/JCM.42.4.1797-1799.2004.

Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea

Annamaria Pratelli  1 , Nicola Decaro, Antonella Tinelli, Vito Martella, Gabriella Elia, Maria Tempesta, Francesco Cirone, Canio Buonavoglia
Affiliations

Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea

Annamaria Pratelli et al. J Clin Microbiol. 2004 Apr.

Abstract

Sixty-nine fecal samples from diarrheic puppies were examined by reverse transcription-PCR assays for the M and the S genes of canine coronaviruses (CCoVs). The isolates in 10 samples were recognized as CCoV type I, and the isolates in 6 samples were recognized as CCoV type II, while isolates of both genotypes were simultaneously detected in 53 samples.

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Figures

FIG. 1.
FIG. 1.
Maximum-parsimony trees constructed with different genome fragments of CCoVs and FCoVs. (a) Fragment of the M gene obtained with primer pair CCoV1a-CCoV2 (239 bp); (b) fragment of the S gene obtained with primer pair EL1F-EL1R (346 bp); (c) fragment of the M gene obtained with primer pair Can1F-CCoV2 (202 bp); (d) fragment of the S gene obtained with primer pair S5-S6 (694 bp). The trees are unrooted and are drawn to scale. Bootstrap values are not shown. The numbers 4, 10, 13, and 19 indicate specimen designations of the PCR products from the fecal samples analyzed.
See this image and copyright information in PMC

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