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. 2004 May;22(5):560-7.
doi: 10.1038/nbt958. Epub 2004 Apr 11.

Adipose-derived adult stromal cells heal critical-size mouse calvarial defects

Catherine M Cowan  1 , Yun-Ying Shi, Oliver O Aalami, Yu-Fen Chou, Carina Mari, Romy Thomas, Natalina Quarto, Christopher H Contag, Benjamin Wu, Michael T Longaker
Affiliations
Free article

Adipose-derived adult stromal cells heal critical-size mouse calvarial defects

Catherine M Cowan et al. Nat Biotechnol. 2004 May.
Free article

Abstract

In adults and children over two years of age, large cranial defects do not reossify successfully, posing a substantial biomedical burden. The osteogenic potential of bone marrow stromal (BMS) cells has been documented. This study investigates the in vivo osteogenic capability of adipose-derived adult stromal (ADAS) cells, BMS cells, calvarial-derived osteoblasts and dura mater cells to heal critical-size mouse calvarial defects. Implanted, apatite-coated, PLGA scaffolds seeded with ADAS or BMS cells produced significant intramembranous bone formation by 2 weeks and areas of complete bony bridging by 12 weeks as shown by X-ray analysis, histology and live micromolecular imaging. The contribution of implanted cells to new bone formation was 84-99% by chromosomal detection. These data show that ADAS cells heal critical-size skeletal defects without genetic manipulation or the addition of exogenous growth factors.

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