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. 2004 May 14;317(4):1138-43.
doi: 10.1016/j.bbrc.2004.03.171.

The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase

Tomoko Andoh  1 , Abul Kalam Azad, Asako Shigematsu, Yasumi Ohshima, Tokio Tani
Affiliations

The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase

Tomoko Andoh et al. Biochem Biophys Res Commun. .

Abstract

Fission yeast ptr1-1 is one of the mRNA transport mutants that accumulate poly(A)+ RNA in the nuclei at the nonpermissive temperature. We found that the ptr1+ gene encodes a homolog of Saccharomyces cerevisiae Tom1p, a hect type ubiquitin ligase. In ptr1-1, a conserved amino acid in the hect domain of Ptr1p is mutated. The ptr1+ gene is essential for growth and its mutation did not affect nuclear protein export. A ptr1-1 rae1-167 double mutant showed a synthetic effect on a growth defect, indicating that Ptr1p functionally interacts with an essential mRNA export factor Rae1p. We also isolated a multi-copy suppressor for ptr1-1 and found that it is the mpd2+ gene isolated as a multi-copy suppressor of cdc7-PD1.

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