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. 2004 Mar;10(3):530-2.
doi: 10.3201/eid1003.030493.

Immunofluorescence assay for serologic diagnosis of SARS

Paul K S Chan  1 , King-Cheung Ng, Rickjason C W Chan, Rebecca K Y Lam, Viola C Y Chow, Mamie Hui, Alan Wu, Nelson Lee, Florence H Y Yap, Frankie W T Cheng, Joseph J Y Sung, John S Tam
Affiliations

Immunofluorescence assay for serologic diagnosis of SARS

Paul K S Chan et al. Emerg Infect Dis. 2004 Mar.

Abstract

We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

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