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. 2004 May 1;10(9):1246-9.
doi: 10.3748/wjg.v10.i9.1246.

Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies

Si-Jin Liu  1 , Zi-Wei Cai, Ya-Jun Liu, Mei-Yu Dong, Li-Qiu Sun, Guo-Fa Hu, Ying-Yun Wei, Wei-De Lao
Affiliations

Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies

Si-Jin Liu et al. World J Gastroenterol. .

Abstract

Aim: To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.

Methods: RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.

Results: We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells. All detected malignant tumor tissues, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.

Conclusion: Nucleostemin can help regulate the proliferation of both cancer cells and stem cells. It might play an important role in the growth regulation of gastric cancer, liver cancer and other cancers.

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Figures

Figure 1
Figure 1
Molecular cloning of nucleostemin cDNA fragment. 1, DNA marker: λDNA, EcoRI + Hind III; 2, pGEM-T-NS, BamHI + Hind III.
Figure 2
Figure 2
Detection of NS expression in various tumor tissues and normal human adult kidney tissues using RT-PCR. 1, GAPDH cDNA-fragment sample; 2, NS-cDNA fragment sample; 3, Renal carcinoma tissue; 4, Normal human adult kid-ney tissue; 5, Bladder carcinoma tissue; 6, Liver cancer tissue; 7, Gastric adenocarcinoma tissue; 8, DNA marker: DNA, EcoR I + Hind III.
Figure 3
Figure 3
Detection of NS expression in various tumor tissues and normal human adult kidney tissues by Slot blot. 1, Liver cancer tissue; 2, Bladder carcinoma tissue; 3, Pancreatic cancer tissue; 4, Cardia adenocarcinoma tissue; 5, Gastric adenocarcinoma tissue; 6, Esophagus squamous carcinoma tissue; 7, Denatured NS-cDNA sample; 8, Ovary serous cystadenocarcinoma; 9, Ampulle carci-noma tissue; 10, Renal carcinoma tissue; 11, Normal human adult kidney tissue; 12, Benign hyperplasia tissue of prostate.
Figure 4
Figure 4
Detection of NS expression in various tumor tissues and normal human adult kidney tissues by Northern blot. 1, Gastric adenocarcinoma tissue; 2, Renal carcinoma tissue; 3, Normal human adult kidney tissue; 4, Liver cancer tissue; 5, Bladder carcinoma tissue.
Figure 5
Figure 5
In situ hybridization detection of nucleostemin mRNA in invasive ductal breast carcinoma and normal mammary gland tissue by SP-HRP method. Counterstained with hematoxylin. A: Ductal carcinoma cells showed strong staining in situ (arrows); B: Normal mammary glandular epithelial cells showed no posi-tive staining, only with azury nuclear counterstaining (arrows).
See this image and copyright information in PMC

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