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. 2004 May 1;10(9):1256-61.
doi: 10.3748/wjg.v10.i9.1256.

Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population

Bao-Ying Fei  1 , Bing Xia, Chang-Sheng Deng, Xiao-Qing Xia, Min Xie, J Bart A Crusius, A Salvador Pena
Affiliations

Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population

Bao-Ying Fei et al. World J Gastroenterol. .

Abstract

Aim: To investigate the association of TNF polymorphisms with chronic atrophic gastritis (CAG) and gastric adenocarcinoma in Chinese Han patients.

Methods: The TNFa-e 5 microsatellites and 3 RFLP sites were typed using PCR technique, followed by high-voltage denaturing PAGE with silver staining and restriction enzyme digestion respectively in specimens from 53 patients with CAG and 56 patients with gastric adenocarcinoma and 164 healthy controls. The PCR products were cloned and sequenced.

Results: The frequency of TNF-beta Ncol*1/2 genotype was higher in patients with chronic atrophic gastritis than in healthy controls, but no significant difference was observed (60.38% vs 46.34%, P=0.076). The frequency of TNa10 allele was significantly higher in patients with chronic atrophic gastritis than in healthy controls (19.81% vs 11.89%, P=0.04). However, it did not relate to age, gender, atrophic degree or intestinal metaplasia in patients with chronic atrophic gastritis. The frequency of TNF-beta Ncol*1/2 and d2/d6 genotypes were significantly higher in patients with gastric adenocarcinoma than in healthy individuals (P>0.05). However, TNF-beta Ncol*1/2 and d2/d6 genotypes did not relate to age, gender, grade of differentiation and clinicopathologic stage in patients with gastric adenocarcinoma. The frequency of TNFa6b5c1 haplotype homozygote was significantly lower in patients with gastric adenocarcinoma than in healthy controls (1.79% vs 15.85%, P=0.006).

Conclusion: TNFa10 allele may be a risk factor for chronic atrophic gastritis. TNF-beta Ncol*1/2 and d2/d6 genotypes are associated with the susceptibility to gastric adenocarcinoma, whereas TNFa6b5c1 haplotype homozygote may contribute to the resistance against gastric adenocarcinoma.

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Figures

Figure 1
Figure 1
Nucleotide sequence of the cloned PGEM-T vector comprising TNFa6 allele (135→241) and TNFb5 allele (29→157), 150→179 fragment is 15 (AC/GT) repeats, 107→127 fragment is 10.5 (TC/GA) repeats.
Figure 2
Figure 2
Nucleotide sequence of the cloned PGEM-T vector comprising TNFc1 allele (35→193), 59→76 fragment is 9 (TC/GA) repeats.
Figure 3
Figure 3
Nucleotide sequence of the cloned PGEM-T vector comprising TNFd4 allele (34→163), 64→85 fragment is 11 (TC/GA) repeats.
Figure 4
Figure 4
Nucleotide sequence of the cloned PGEM-T vector comprising TNFe3 allele (29→131), 47→62 fragment is 8 (TC/GA) repeats.
See this image and copyright information in PMC

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