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Review
. 2004;5(5):223.
doi: 10.1186/gb-2004-5-5-223. Epub 2004 Apr 16.

Maize DNA-sequencing strategies and genome organization

Ron J Okagaki  1 , Ronald L Phillips
Affiliations
Review

Maize DNA-sequencing strategies and genome organization

Ron J Okagaki et al. Genome Biol. 2004.

Abstract

A large amount of repetitive DNA complicates the assembly of the maize genome sequence. Genome-filtration techniques, such as methylation-filtration and high-CoT separation, enrich gene sequences in genomic libraries. These methods may provide a low-cost alternative to whole-genome sequencing for maize and other complex genomes.

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References

    1. Bennetzen JL, Chandler VL, Schnable P. National Science Foundation-sponsored workshop report. Maize genome sequencing project. Plant Physiol. 2001;127:1572–1578. doi: 10.1104/pp.127.4.1572. - DOI - PMC - PubMed
    1. Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al. Initial sequencing and analysis of the human genome. Nature. 2001;409:860–921. doi: 10.1038/35057062. - DOI - PubMed
    1. SanMiguel P, Gaut BS, Tikhonov A, Nakajima Y, Bennetzen JL. The paleontology of intergene retrotransposons of maize. Nat Genet. 1998;20:43–45. doi: 10.1038/1695. - DOI - PubMed
    1. The Arabidopsis Genome Initiative Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000;408:796–815. doi: 10.1038/35048692. - DOI - PubMed
    1. Yu J, Hu S, Wang J, Wong GK-S, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, et al. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science. 2002;296:79–92. doi: 10.1126/science.1068037. - DOI - PubMed

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