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. 2004 May;42(5):2218-20.
doi: 10.1128/JCM.42.5.2218-2220.2004.

Development and evaluation of a new commercial test allowing the simultaneous detection of noroviruses and sapoviruses by reverse transcription-PCR and microplate hybridization

F Bon  1 , H Giraudon, C Sancey, C Barranger, M Joannes, P Pothier, E Kohli
Affiliations

Development and evaluation of a new commercial test allowing the simultaneous detection of noroviruses and sapoviruses by reverse transcription-PCR and microplate hybridization

F Bon et al. J Clin Microbiol. 2004 May.

Abstract

This work describes the design and initial evaluation of a commercial test allowing the detection of noroviruses and sapoviruses by reverse transcription-PCR (RT-PCR) in a single tube followed by microplate hybridization, as well as the detection of PCR inhibitors. The test was shown to be broadly reactive (except for Melksham-like strains), sensitive, and specific and thus should be useful for calicivirus detection in clinical practice.

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References

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