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. 2004 Jun;57(3):415-20.
doi: 10.1016/j.mimet.2004.01.015.

Fast screening method for detection of acyl-HSL-degrading soil isolates

Sylwia Jafra  1 , Jean Martin van der Wolf
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Fast screening method for detection of acyl-HSL-degrading soil isolates

Sylwia Jafra et al. J Microbiol Methods. 2004 Jun.

Abstract

A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc)).

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