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. 2004 May 20;100(1-2):11-6.
doi: 10.1016/S0378-1135(03)00126-3.

Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates

Christoph G Baums  1 , Ulrich Schotte, Gunter Amtsberg, Ralph Goethe
Affiliations

Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates

Christoph G Baums et al. Vet Microbiol. .

Abstract

In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C. perfringens reference strains and isolates from veterinary bacteriological routine diagnostic specimens.

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