Preimplantation genetic diagnosis for the treatment of failed in vitro fertilization-embryo transfer and habitual abortion

Abstract

Objective: To apply preimplantation genetic diagnosis (PGD) for the treatment of patients with a history of failed IVF-ET or habitual aborters.

Design: Prospective clinical study.

Setting: Tertiary center for assisted reproduction.

Patient(s): Ninety-four couples with failed IVF-ET after >2 IVF cycles and 64 couples with >2 spontaneous abortions.

Intervention(s): Patients were prepared for oocyte retrieval using standard controlled ovarian hyperstimulation protocols after standard laboratory techniques. Blastomeres from 6- to 8-cell embryos were analysed using fluorescence in situ hybridization with commercial chromosomal probes, and normoploid embryos were transferred on day 3 after fertilization.

Main outcome measure(s): Pregnancy and implantation rates and live births.

Result(s): Both 3- and 5-probe PGD resulted in a significantly higher outcome than controls for failed IVF-ET. Five-probe PGD appeared to be more suitable for habitual aborters.

Conclusion(s): This pilot study suggests that 3-probe PGD is a valid option for failed IVF-ET patients. The use of five or more probes is indicated for habitual aborters.