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. 2004 May;54(Pt 3):689-691.
doi: 10.1099/ijs.0.02888-0.

Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov

Karen Vermis  1 , Tom Coenye  2 , John J LiPuma  3 , Eshwar Mahenthiralingam  4 , Hans J Nelis  1 , Peter Vandamme  2
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Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov

Karen Vermis et al. Int J Syst Evol Microbiol. 2004 May.

Abstract

Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.

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