Living bacterial cell array for genotoxin monitoring

Abstract

A biosensor composed of a high-density living bacterial cell array was fabricated by inserting bacteria into a microwell array formed on one end of an imaging fiber bundle. The size of each microwell allows only one cell to occupy each well. In this biosensor, E. coli cells carrying a recA::gfp fusion were used as sensing components for genotoxin detection. Each fiber in the array has its own light pathway, enabling thousands of individual cell responses to be monitored simultaneously with both spatial and temporal resolution. The biosensor was capable of performing cell-based functional sensing of a genotoxin with high sensitivity and short incubation times (1 ng/mL mitomycin C after 90 min). Dose-response curves for several genotoxins were obtained. The biosensors demonstrated an active sensing lifetime of more than 6 h and a shelf lifetime of two weeks.