Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography
- PMID: 15146918
- DOI: 10.1016/j.chroma.2004.02.073
Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography
Abstract
High-speed counter-current chromatography was used to isolate and purify honokiol and magnolol from cortex Magnoliae Officinalis (Magnolia officinalis Rehd. et Wils.), a plant used in the traditional Chinese medicine. A crude sample, 150 mg, was successfully separated with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.4:1:0.4, v/v), and the fractions were analyzed by high-performance liquid chromatography. The separation produced 80 and 45 mg of honokiol and magnolol with purities of 99.2 and 98.2%, respectively, in 2.5 h.
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