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. 1992;104(6):359-60.
doi: 10.1007/BF01369558.

DNA extraction from mixtures of body fluid using mild preferential lysis

P Wiegand  1 , M Schürenkamp, U Schütte
Affiliations

DNA extraction from mixtures of body fluid using mild preferential lysis

P Wiegand et al. Int J Legal Med. 1992.

Abstract

A modification to the DNA extraction method "preferential lysis" (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using "mild preferential lysis". Amplification by PCR (polymerase chain reaction) then yields sufficient DNA to be able to identify both components in the mixture.

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