doi: 10.1016/j.bbadis.2004.02.003.
The prion-like protein Doppel fails to interact with itself, the prion protein and the 37 kDa/67 kDa laminin receptor in the yeast two-hybrid system
Affiliations
- PMID: 15158907
- DOI: 10.1016/j.bbadis.2004.02.003
Item in Clipboard
The prion-like protein Doppel fails to interact with itself, the prion protein and the 37 kDa/67 kDa laminin receptor in the yeast two-hybrid system
Biochim Biophys Acta.
.
Free article
Abstract
The prion-like protein termed Doppel (Dpl) shows approx. 25% sequence identity with all known prion proteins (PrP). We recently showed that the cellular PrP is dimeric under native conditions, a finding which was confirmed by the investigation of its crystal structure. Human PrP further interacts with its cellular receptor, the 37 kDa/67 kDa laminin receptor (LRP/LR). Here we report that human Doppel fails to interact with (i). itself, (ii). the human 37 kDa/67 kDa LRP/LR, and (iii). the human cellular prion protein (huPrP) in the yeast two-hybrid system. Our findings suggest that Dpl and PrP are not related or are only marginally related with respect to their ligand binding behaviour.
Similar articles
-
Identification of interaction domains of the prion protein with its 37-kDa/67-kDa laminin receptor.EMBO J. 2001 Nov 1;20(21):5876-86. doi: 10.1093/emboj/20.21.5876. EMBO J. 2001. PMID: 11689428 Free PMC article.
-
Interaction of Doppel with the full-length laminin receptor precursor protein.Arch Biochem Biophys. 2004 Aug 15;428(2):165-9. doi: 10.1016/j.abb.2004.06.003. Arch Biochem Biophys. 2004. PMID: 15246873
-
The 37-kDa/67-kDa laminin receptor acts as the cell-surface receptor for the cellular prion protein.EMBO J. 2001 Nov 1;20(21):5863-75. doi: 10.1093/emboj/20.21.5863. EMBO J. 2001. PMID: 11689427 Free PMC article.
-
Interactions between PrP(c) and other ligands with the 37-kDa/67-kDa laminin receptor.Front Biosci (Landmark Ed). 2010 Jun 1;15(3):1150-63. doi: 10.2741/3667. Front Biosci (Landmark Ed). 2010. PMID: 20515747 Review.
-
Role of the 37 kDa laminin receptor precursor in the life cycle of prions.Transfus Clin Biol. 1999 Feb;6(1):7-16. doi: 10.1016/S1246-7820(99)80006-8. Transfus Clin Biol. 1999. PMID: 10188208 Review.
Cited by
-
Doppel and PrPC co-immunoprecipitate in detergent-resistant membrane domains of epithelial FRT cells.Biochem J. 2009 Dec 23;425(2):341-51. doi: 10.1042/BJ20091050. Biochem J. 2009. PMID: 19888917 Free PMC article.
-
Interactome analyses identify ties of PrP and its mammalian paralogs to oligomannosidic N-glycans and endoplasmic reticulum-derived chaperones.PLoS Pathog. 2009 Oct;5(10):e1000608. doi: 10.1371/journal.ppat.1000608. Epub 2009 Oct 2. PLoS Pathog. 2009. PMID: 19798432 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
