Molecular targets and mechanisms in formation of chromosomal aberrations: contributions of Soviet scientists

Abstract

Studies of mechanisms for formation of chromosomal aberrations (CAs) with special emphasis on data from Soviet/Russian investigations are reviewed that argue in favor of a minor fraction of genomic DNA that forms specific molecular targets/contacts for the formation of chromosomal exchanges. This DNA is presumably associated with matrix attachment sites of DNA loops, enriched with AT base pairs and repetitive DNA sequences. It is assumed that there are two main mechanisms in formation of chromosome aberrations: 1) mutually reciprocal recombination, resulting in formation of all kinds of chromosome exchanges; 2) the process of telomere formation, resulting in the generation of true deletions. A significant part of chromosomal breaks and apparently unrejoined ends in incomplete exchanges as seen with cytogenetic techniques reflect decondensation in the discrete units of chromatin organization such as the megabase-size DNA domains. The possible ways for further analysis of alternative theories with emerging technologies are also discussed.