Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jul;83(7):684-7.
doi: 10.1111/j.1349-7006.1992.tb01966.x.

Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines

P Russo  1 , S Parodi, G Billi, C Oliva, M Venturini, E Noviello, P Conte
Affiliations

Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines

P Russo et al. Jpn J Cancer Res. 1992 Jul.

Abstract

The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PA1), while 3 cell lines (IGROV1, SKOV3, Me180) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. ) Old , L.Tumor necrosis factor (rTNF) . Science , 230 , 630 – 636 ( 1985. ). - PubMed
    1. ) Balkwill , F. R. , Naylor , M. S. and Malik , S.Tumor necrosis factor as an anticancer agent . Eur. J. Cancer , 26 , 641 – 644 ( 1990. ). - PubMed
    1. ) Alexander , R. B. , Nelson , W. G. and Coffey , D. S.Synergistic enhancement by tumor necrosis factor of in vitro cytotoxicity from chemotherapeutic drugs targeted at DNA topoisomerase II . Cancer Res. , 47 , 2403 – 2406 ( 1987. ). - PubMed
    1. ) Utsugy , T. , Mattern , M. R. , Mirabelli , C. K. and Hanna , N.Potentiation of topoisomerase inhibitor‐induced DNA strand breakage and cytotoxicity by tumor necrosis factor: enhancement of topoisomerase activity as a mechanism of potentiation . Cancer Res. , 50 , 2636 – 2640 ( 1990. ). - PubMed
    1. ) Coffman , F. D. , Green , L. M. , Godwin , A. and Ware , C. F.Cytotoxicity mediated by tumor necrosis factor in variant subclones of Me‐180 cervical carcinoma line: modulation by specific inhibitors of DNA topoisomerase II . J. Cell. Biochem. , 39 , 95 – 105 ( 1989. ). - PubMed

Publication types

MeSH terms

LinkOut - more resources