Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004:284:37-49.
doi: 10.1385/1-59259-816-1:037.

Two-dimensional gel electrophoresis for the identification of signaling targets

Yukihito Kabuyama  1 , Kirsi K Polvinen, Katheryn A Resing, Natalie G Ahn
Affiliations

Two-dimensional gel electrophoresis for the identification of signaling targets

Yukihito Kabuyama et al. Methods Mol Biol. 2004.

Abstract

Two-dimensional electrophoresis (2-DE) is a powerful technique to differentially display patterns of protein expression and posttranslational modifications, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme.
    Kabuyama Y, Litman ES, Templeton PD, Metzner SI, Witze ES, Argast GM, Langer SJ, Polvinen K, Shellman Y, Chan D, Shabb JB, Fitzpatrick JE, Resing KA, Sousa MC, Ahn NG. Kabuyama Y, et al. Mol Cell Proteomics. 2009 Oct;8(10):2308-20. doi: 10.1074/mcp.M900178-MCP200. Epub 2009 Jul 20. Mol Cell Proteomics. 2009. PMID: 19620624 Free PMC article.

LinkOut - more resources