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. 2004 Jul 2;319(3):817-25.
doi: 10.1016/j.bbrc.2004.05.057.

T47D breast cancer cell growth is inhibited by expression of VACM-1, a cul-5 gene

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T47D breast cancer cell growth is inhibited by expression of VACM-1, a cul-5 gene

Maria A Burnatowska-Hledin et al. Biochem Biophys Res Commun. .

Abstract

Vasopressin-activated calcium-mobilizing (VACM-1), a cul-5 gene, is localized on chromosome 11q22-23 close to the gene for Ataxia Telangiectasia in a region associated with a loss of heterozygosity in breast cancer tumor samples. To examine the biological role of VACM-1, we studied the effect of VACM-1 expression on cellular growth and gene expression in T47D breast cancer cells. Immunocytochemistry studies demonstrated that VACM-1 was expressed in 0.6-6% of the T47D cells and localized to the nucleus of mitotic cells. Overexpressing VACM-1 significantly attenuated cellular proliferation and MAPK phosphorylation when compared to the control cells. In addition, VACM-1 decreased egr-1 and increased Fas-L mRNA levels. Further, egr-1 protein levels were significantly lower in the nuclear fraction from VACM-1 transfected cells when compared to controls. These data indicate that VACM-1 is involved in the regulation of cellular growth.

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