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Comparative Study
. 2004 Jun;70(6):3733-5.
doi: 10.1128/AEM.70.6.3733-3735.2004.

Methods for enhanced culture recovery of Francisella tularensis

Jeannine M Petersen  1 , Martin E Schriefer, Kenneth L Gage, John A Montenieri, Leon G Carter, Miles Stanley, May C Chu
Affiliations
Comparative Study

Methods for enhanced culture recovery of Francisella tularensis

Jeannine M Petersen et al. Appl Environ Microbiol. 2004 Jun.

Abstract

Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, antibiotic supplementation of enriched cysteine heart agar blood culture medium improved recovery of F. tularensis by 81.1%. For transport of tissues, immediate freezing yielded culture recovery rates as high as 94%.

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Figures

FIG. 1.
FIG. 1.
Comparison of carcass tissues plated on CHAB (left panel) and on CHAB-A (right panel).
See this image and copyright information in PMC

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