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. 2004 Jun;70(6):3769-71.
doi: 10.1128/AEM.70.6.3769-3771.2004.

Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

Mohd Amir F Abdullah  1 , Donald H Dean
Affiliations

Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

Mohd Amir F Abdullah et al. Appl Environ Microbiol. 2004 Jun.

Abstract

Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.

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Figures

FIG. 1.
FIG. 1.
Ribbon representation of model structures of Cry4Ba (left) and Cry19Aa (right). The arrows indicate the positions of the putative loop regions of domain II.
FIG. 2.
FIG. 2.
Sequence alignments based on the model structures made with Swiss-Pdb Viewer of Cry4Ba with Cry19Aa. Putative loop positions are indicated on top of the sequences, while the amino acid residues that were mutated are underlined.
FIG. 3.
FIG. 3.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of high-pressure liquid chromatography-purified non-trypsin-activated and trypsin-activated toxins. Lanes: 1, marker; 2, Cry19Aa, non-trypsin activated; 3, Cry19AL1L2, non-trypsin activated; 4, 19Aa, trypsin activated; 5, 19AL1L2, trypsin activated.
See this image and copyright information in PMC

References

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    1. Peitsch, M. C. 1996. ProMod and Swiss-Model: internet-based tools for automated comparative protein modelling. Biochem. Soc. Trans. 24:274-279. - PubMed

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