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. 2004 Jun;42(6):2682-4.
doi: 10.1128/JCM.42.6.2682-2684.2004.

Prevalence of Helicobacter pylori vacA and cagA genotypes in Ethiopian dyspeptic patients

Daniel Asrat  1 , Ingrid Nilsson, Yohannes Mengistu, Endale Kassa, Senait Ashenafi, Kiros Ayenew, Torkel Wadström, Waleed Abu-Al-Soud
Affiliations

Prevalence of Helicobacter pylori vacA and cagA genotypes in Ethiopian dyspeptic patients

Daniel Asrat et al. J Clin Microbiol. 2004 Jun.

Abstract

A total of 300 gastric biopsy samples and 50 Helicobacter pylori isolates were collected from Ethiopian adult dyspeptic patients. The vacA and cagA genes were detected in 90 and 79% of biopsy specimens, respectively, and in 100 and 87% of clinical isolates, respectively. Both genes were detected in 84% of the gastric biopsy samples and in 87% of the clinical isolates. Among vacA genotypes, the s1/m1 genotype was the most common in gastric biopsy samples (48%). The vacA and cagA positive H. pylori strains were detected to a higher degree in patients with chronic active gastritis (71%) than patients with other histopathological findings (29%) (P < 0.05).

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Figures

FIG. 1.
FIG. 1.
DGGE analysis of PCR products amplified by using Helicobacter genus-specific primers. Lanes, 1 to 4 and 6 to 22, H. pylori-positive samples; 5, H. pylori-negative sample; M, mobility marker (PCR products of reference Helicobacter spp. [from the top: H. bilis, H. pullorum, H. pylori, “F. rappini,” H. hepaticus, and H. bizozzeroni]).
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