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Comparative Study
. 2004;5(6):R42.
doi: 10.1186/gb-2004-5-6-r42. Epub 2004 May 18.

Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis

Brian W Brunelle  1 , Tracy L Nicholson, Richard S Stephens
Affiliations
Comparative Study

Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis

Brian W Brunelle et al. Genome Biol. 2004.

Abstract

By comparing two fully sequenced genomes of Chlamydia trachomatis using competitive hybridization on DNA microarrays, a logarithmic correlation was demonstrated between the signal ratio of the arrays and the 75-99% range of nucleotide identities of the genes. Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis.

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Figures

Figure 1
Figure 1
Frequency of gene pair identities between MoPn and D/UW-3. The percent identity for each orthologous gene pair between C. trachomatis serovars D/UW-3 and MoPn was established and rounded to the nearest whole number. The number of times each nucleotide identity occurred was then determined.
Figure 2
Figure 2
Relationship between the signal ratios and sequence identities for the MoPn vs D/UW-3 DNA microarray. The average signal ratio of each orthologous gene pair between C. trachomatis serovars D/UW-3 and MoPn was log2 transformed. These values were then compared to the corresponding nucleotide identity for each gene pair, yielding a linear association.
See this image and copyright information in PMC

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