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. 2004 Jun 11;304(5677):1672-5.
doi: 10.1126/science.1099366.

Phosphoryl transfer and calcium ion occlusion in the calcium pump

Thomas Lykke-Møller Sørensen  1 , Jesper Vuust Møller, Poul Nissen
Affiliations

Phosphoryl transfer and calcium ion occlusion in the calcium pump

Thomas Lykke-Møller Sørensen et al. Science. .

Abstract

A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane.

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