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. 2004:6:113-119.
doi: 10.1251/bpo79. Epub 2004 Jun 8.

Using C. elegans to screen for targets of ethanol and behavior-altering drugs

Andrew G. Davies  1 , Steven L. McIntire
Affiliations

Using C. elegans to screen for targets of ethanol and behavior-altering drugs

Andrew G. Davies et al. Biol Proced Online. 2004.

Abstract

Caenorhabditis elegans is an attractive model system for determining the targets of neuroactive compounds. Genetic screens in C. elegans provide a relatively unbiased approach to the identification of genes that are essential for behavioral effects of drugs and neuroactive compounds such as alcohol. Much work in vertebrate systems has identified multiple potential targets of ethanol but which, if any, of those candidates are responsible for the behavioral effects of alcohol is uncertain. Here we provide detailed methodology for a genetic screen for mutants of C. elegans that are resistant to the depressive effects of ethanol on locomotion and for the subsequent behavioral analysis of those mutants. The methods we describe should also be applicable for use in screening for mutants that are resistant or hypersensitive to many neuroactive compounds and for identifying the molecular targets or biochemical pathways mediating drug responses.

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Figures

Fig. 1
Fig. 1. Alternative strategies for screening for resistance to the depressive effects of ethanol on locomotion that use either (A) a food source or (B) an attractive odorant to encourage movement.
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