Single-molecule manipulation of nucleic acids
- PMID: 15193318
- DOI: 10.1016/j.sbi.2004.03.016
Single-molecule manipulation of nucleic acids
Abstract
During the past decade, local force measurement techniques, such as atomic force microscopy and optical tweezers, were used to study the elastic properties and mechanically induced structural transitions of nucleic acids at the single-molecule level. Single-molecule manipulation has also increasingly been used to investigate DNA-dependent enzymatic processes, with implications for unfolding and modifying DNA, protein-DNA interactions, replication and transcription. Compared to classical techniques of molecular biology, single-molecule measurements avoid the need to average over a large number of events, and can thus potentially provide detailed and complementary information.
Similar articles
-
Mechanisms of DNA binding determined in optical tweezers experiments.Biopolymers. 2007 Feb 5;85(2):154-68. doi: 10.1002/bip.20622. Biopolymers. 2007. PMID: 17080421 Review.
-
[Molecular construction (superstructures) with adjustable properties based on double-stranded nucleic acids].Mol Biol (Mosk). 2003 Mar-Apr;37(2):340-55. Mol Biol (Mosk). 2003. PMID: 12723480 Review. Russian.
-
[Biophysics of the DNA molecule: a new trend].Mol Biol (Mosk). 2002 Mar-Apr;36(2):307-11. Mol Biol (Mosk). 2002. PMID: 11969092 Russian.
-
Protein unfolding and refolding under force: methodologies for nanomechanics.Chemphyschem. 2005 Jan;6(1):29-34. doi: 10.1002/cphc.200400343. Chemphyschem. 2005. PMID: 15688640 Review.
-
Single DNA molecule analysis of transcription complexes.Methods Enzymol. 2003;371:34-50. doi: 10.1016/S0076-6879(03)71003-9. Methods Enzymol. 2003. PMID: 14712690 No abstract available.
Cited by
-
DNA overstretching transition: ionic strength effects.Biophys J. 2005 Aug;89(2):967-78. doi: 10.1529/biophysj.105.063099. Epub 2005 May 27. Biophys J. 2005. PMID: 15923227 Free PMC article.
-
Electron Paramagnetic Resonance of a Single NV Nanodiamond Attached to an Individual Biomolecule.Biophys J. 2016 May 10;110(9):2044-52. doi: 10.1016/j.bpj.2016.03.022. Biophys J. 2016. PMID: 27166812 Free PMC article.
-
Dynamics of the DNA duplex formation studied by single molecule force measurements.Biophys J. 2004 Nov;87(5):3388-96. doi: 10.1529/biophysj.104.039776. Epub 2004 Aug 31. Biophys J. 2004. PMID: 15339815 Free PMC article.
-
Strain softening in stretched DNA.Phys Rev Lett. 2008 Sep 12;101(11):118101. doi: 10.1103/PhysRevLett.101.118101. Epub 2008 Sep 10. Phys Rev Lett. 2008. PMID: 18851334 Free PMC article.
-
Quantitative modeling and optimization of magnetic tweezers.Biophys J. 2009 Jun 17;96(12):5040-9. doi: 10.1016/j.bpj.2009.03.055. Biophys J. 2009. PMID: 19527664 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous