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. 2004 Aug 5;807(2):217-21.
doi: 10.1016/j.jchromb.2004.04.011.

Determination of rofecoxib in human plasma and breast milk by high-performance liquid chromatographic assay

Mei Zhang  1 , Grant A Moore, Sharon J Gardiner, Evan J Begg
Affiliations

Determination of rofecoxib in human plasma and breast milk by high-performance liquid chromatographic assay

Mei Zhang et al. J Chromatogr B Analyt Technol Biomed Life Sci. .

Abstract

A rapid and simple HPLC assay was developed for the determination of rofecoxib in human plasma and breast milk. After solid-phase extraction, rofecoxib was resolved on a C18 column and detected by UV detection at 272 nm. Standard curves were linear over the concentration range 10-2000 microg/L (r2 >0.99). Intra- and inter-day coefficients of variation for both matrices were <10% and the limit of quantification was around 10 microg/L.

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