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. 2004 Jul;88(7):947-9.
doi: 10.1136/bjo.2003.024646.

Activation of nuclear factor-kappa B in the conjunctiva with the epithelial scraping of the mouse cornea and human epidemic keratoconjunctivitis

S Kase  1 , K Aoki, T Harada, C Harada, K Ohgami, K Shiratori, S Nishi, S Ohno, K Yoshida
Affiliations

Activation of nuclear factor-kappa B in the conjunctiva with the epithelial scraping of the mouse cornea and human epidemic keratoconjunctivitis

S Kase et al. Br J Ophthalmol. 2004 Jul.

Abstract

Aim: To examine the expression of p65, one of nuclear factor-kappa B (NF-kappa B), in the conjunctival epithelium of the C57Bl6 mouse and a patient with epidemic keratoconjunctivitis (EKC).

Methods: Normal and epithelial scraped cornea obtained 6 hours after the injury were processed for paraffin section. Samples of a normal and an EKC conjunctival epithelium were obtained using impression cytology. Both samples were analysed by immunocytochemistry using anti-p65 antibody.

Results: Immunocytochemistry with the anti-NF-kappa B p65 antibody revealed that p65 was localised in the cytoplasm of the conjunctival epithelium in the C57Bl6 mouse without the treatment. Six hours after the scraping of the cornea, p65 protein was expressed in the nuclei of the conjunctival epithelium. p65 was localised in the cytoplasm of the conjunctival epithelium in the human normal eye. p65 protein was expressed in the nuclei of the conjunctival epithelial cells in the EKC patient.

Conclusion: These findings suggest that NF-kappa B was activated in the conjunctiva in the epithelial scraping of the mouse cornea and in human EKC.

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Figures

Figure 1
Figure 1
Immunodetection of NF-κB p65 protein in the conjunctiva without treatment (A) and 6 hours after the epithelial scraping of the cornea (B) in the C57Bl6 mouse. (A) p65 protein is localised in the cytoplasm of the conjunctival epithelial cells without treatment. (B) In contrast, nuclear immunoreactivity for p65 protein is observed in the epithelium of the conjunctiva after epithelial scraping of the cornea (magnification ×40).
Figure 2
Figure 2
Immunodetection of p65 protein in the palpebral conjunctiva from the normal human (A) and epidemic keratoconjunctivitis (EKC) patient (B). An impression cytology technique was adopted for immunocytological analysis of the conjunctival epithelium. (A) Immunoreactivity was noted in the cytoplasm of squamous epithelial cells in a normal mucosa. (B) In contrast, nuclear immunoreactivity for p65 was noted in the squamous epithelium in an EKC patient (magnification, ×60).
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