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. 2004 Jul;186(13):4412-6.
doi: 10.1128/JB.186.13.4412-4416.2004.

The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class A penicillin-binding protein

Colette Duez  1 , Séverine Hallut, Noureddine Rhazi, Séverine Hubert, Ana Amoroso, Fabrice Bouillenne, André Piette, Jacques Coyette
Affiliations

The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class A penicillin-binding protein

Colette Duez et al. J Bacteriol. 2004 Jul.

Abstract

A soluble derivative of the Enterococcus faecalis JH2-2 class A PBP1 (*PBP1) was overproduced and purified. It exhibited a glycosyltransferase activity on the Escherichia coli 14C-labeled lipid II precursor. As a DD- peptidase, it could hydrolyze thiolester substrates with efficiencies similar to those of other class A penicillin-binding proteins (PBPs) and bind beta-lactams, but with k2/K (a parameter accounting for the acylation step efficiency) values characteristic of penicillin-resistant PBPs.

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