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. 1992 Jun;74(6):581-4.
doi: 10.1016/0300-9084(92)90158-b.

Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of E coli

Y Nakamura  1 , K Kawakami
Affiliations

Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of E coli

Y Nakamura et al. Biochimie. 1992 Jun.

Abstract

Lysyl-tRNA synthetases are synthesized from two distinct genes in E coli, lysS and lysU, but neither gene product has been purified distinctively by using overproducing systems. The lysS gene has been identified by a herC mutation which restores maintenance of the mutant ColE1 replicon. The herC gene product was overproduced by using a tac promoter fusion and purified to homogeneity. The purified HerC protein possesses a lysyl-tRNA synthetase activity as predicted by the sequence identity of herC to lysS. The procedure is useful for rapid mass-scale purification of lysyl-tRNA synthetase.

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