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. 2004 Jul;48(7):2742-6.
doi: 10.1128/AAC.48.7.2742-2746.2004.

In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay

Abdalla O A Ahmed  1 , Wendy W J van de Sande, Wim van Vianen, Alex van Belkum, Ahmed H Fahal, Henri A Verbrugh, Irma A J M Bakker-Woudenberg
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In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay

Abdalla O A Ahmed et al. Antimicrob Agents Chemother. 2004 Jul.

Abstract

Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC(50)]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC(50) of 0.5 to 1.0 mg/liter).

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Figures

FIG. 1.
FIG. 1.
Reproducibility of susceptibility testing of M. mycetomatis strain mm-55 against AMB and ITC by the XTT method. Curves represent the relative extinction at 450 nm for each drug concentration compared to the growth control (100%). Assays were repeated eight times.
FIG. 2.
FIG. 2.
Antifungal susceptibility testing of M. mycetomatis strain mm-55 against AMB and ITC in duplicate. Curves represent the relative extinction at 450 nm for each drug concentration compared to the growth control (100%) obtained by the XTT assay. Bars represent the growth levels obtained by the modified NCCLS method as determined in a separate experiment.
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References

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