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. 2004 Jul 1;32(Web Server issue):W372-4.
doi: 10.1093/nar/gkh374.

MITOPRED: a web server for the prediction of mitochondrial proteins

Chittibabu Guda  1 , Purnima Guda, Eoin Fahy, Shankar Subramaniam
Affiliations

MITOPRED: a web server for the prediction of mitochondrial proteins

Chittibabu Guda et al. Nucleic Acids Res. .

Abstract

MITOPRED web server enables prediction of nucleus-encoded mitochondrial proteins in all eukaryotic species. Predictions are made using a new algorithm based primarily on Pfam domain occurrence patterns in mitochondrial and non-mitochondrial locations. Pre-calculated predictions are instantly accessible for proteomes of Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila, Homo sapiens, Mus musculus and Arabidopsis species as well as all the eukaryotic sequences in the Swiss-Prot and TrEMBL databases. Queries, at different confidence levels, can be made through four distinct options: (i) entering Swiss-Prot/TrEMBL accession numbers; (ii) uploading a local file with such accession numbers; (iii) entering protein sequences; (iv) uploading a local file containing protein sequences in FASTA format. Automated updates are scheduled for the pre-calculated prediction database so as to provide access to the most current data. The server, its documentation and the data are available from http://mitopred.sdsc.edu.

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Figures

Figure 1
Figure 1
Flow diagram of implementation of the MITOPRED server.
Figure 2
Figure 2
A screen shot of prediction results from MITOPRED.
See this image and copyright information in PMC

References

    1. Kumar A., Agarwal,S., Heyman,J.A., Matson,S., Heidtman,M., Piccirillo,S., Umansky,L., Drawid,A., Jansen,R., Liu,Y. et al. (2002) Subcellular localization of the yeast proteome. Genes Dev., 16,707–719. - PMC - PubMed
    1. Huh W.-K., Falvo,J.V., Gerke,L.C., Carroll,A.S., Howson,R.W., Weissman,J.S. and O'Shea,E.K. (2003) Global analysis of protein localization in budding yeast. Nature, 425, 686–691. - PubMed
    1. Nielsen H., Engelbrech,J., Brunak,S. and von Heijne,G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng., 10, 1–6. - PubMed
    1. Fujiwara Y., Asogawa,M. and Nakai,K. (1997) Prediction of mitochondrial targeting signals using hidden Markov models. In Miyano,S. and Takagi,T. (eds), Genome Informatics. Universal Academy Press, Inc., Tokyo, Japan, pp. 53–60. - PubMed
    1. Nakai K. and Horton,P. (1999) PSORT: a program for detecting the sorting signals of proteins and predicting their subcellular localization. Trends Biochem., Sci., 24, 34–36. - PubMed

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